4. Explain the principle of the PT (prothrombin Test), its reference range and which coagulation factors are measured in the test
Prothrombin time test measures prothrombin factor II, it
evaluates the ability of a blood clot. prothrombin time test is a
one-stage test based upon fibrin clot to form in the required time
after adding tissue factors(thromboplastin), phospholipid, and
calcium to do calcified.
PT reference range depends upon the source of tissue factors like
human and animal like a rabbit
Method of point determination like optical and mechanical
The technique used as manual or automated.
PT measures the action of extrinsic and common pathways of
coagulation as per factors VII, V, X, II, and fibrinogen functional
ability.
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