Question

What is the principle of Optochin?

What is the principle of Optochin?

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Answer #1

What is the principle of Optochin?

Ethylhydrocupreine hydrochloride, a chemical name of Optochin - water soluble, used for the detection of Streptococcus pneumoniae which is  alpha-hemolytic Streptococcus susceptible to it. Other species are resistant to optochinl.

This test uses diffusion principle and it is performed in a blood agar medium. A positive identification of streptococcus pneumoniae by showing a impregnated disk surrounded with lysed optochin due to changes in surface tension.

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