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ANA Test which of the different methodologies and principles applies to this test ?

ANA Test

which of the different methodologies and principles applies to this test ?

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Answer #1

Antinuclear antibody (ANA) test a marker of the autoimmune process that is positive with a variety of autoimmune diseases. It may be positive in

  • Systemic lupus erythematosus,
  • Sjögren's syndrome,
  • Rheumatoid arthritis,
  • Autoimmune hepatitis, etc

Methods of detection of ANA

  • Indirect Immuno flourescence- gold standard ( 80%-sensitivity;specificity-90-97%)
  • ELISA ( 99%-sensitivity;specificity-36-94%)
  • Immunodiffusion (outchterlony)
  • CIEP (Counterimmuno-electrophoresis)
  • Multiple immunobead assay

Principle

  • An indirect immunofluorescence technique isutilized where patient samples and appropriatecontrols are incubated with the substrate slides.
  • The unreacted antibodies are washed off and anappropriate fluorescence labeled conjugate isapplied.
  • Unbound conjugate is washed off, and slides areviewed with a fluorescence microscope.
  • Positive samples produce apple-greenfluorescence.
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