Question

ANA Test which of the different methodologies and principles applies to this test ?

ANA Test

which of the different methodologies and principles applies to this test ?

Homework Answers

Answer #1

Antinuclear antibody (ANA) test a marker of the autoimmune process that is positive with a variety of autoimmune diseases. It may be positive in

  • Systemic lupus erythematosus,
  • Sjögren's syndrome,
  • Rheumatoid arthritis,
  • Autoimmune hepatitis, etc

Methods of detection of ANA

  • Indirect Immuno flourescence- gold standard ( 80%-sensitivity;specificity-90-97%)
  • ELISA ( 99%-sensitivity;specificity-36-94%)
  • Immunodiffusion (outchterlony)
  • CIEP (Counterimmuno-electrophoresis)
  • Multiple immunobead assay

Principle

  • An indirect immunofluorescence technique isutilized where patient samples and appropriatecontrols are incubated with the substrate slides.
  • The unreacted antibodies are washed off and anappropriate fluorescence labeled conjugate isapplied.
  • Unbound conjugate is washed off, and slides areviewed with a fluorescence microscope.
  • Positive samples produce apple-greenfluorescence.
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
ANA refers to antinuclear antibodies. What are antinuclear antibodies and what does a positive ANA test...
ANA refers to antinuclear antibodies. What are antinuclear antibodies and what does a positive ANA test tells us?
Consider the use of the ANA (antinuclear antibody) test in the diagnosis of SLE (systemic lupus...
Consider the use of the ANA (antinuclear antibody) test in the diagnosis of SLE (systemic lupus erythematosus). In a rheumatology practice, the prevalence of SLE in patients on whom an ANA test was done was 2.88% with a hypothetical population of 100,000 patients. The sensitivity of the ANA for SLE is 98% and the specificity is 93%. What are the True Positives, True Negatives, PPV, NPV, and percent agreement (unpaired) of the test? (Show all calculations for partial credit)
Which of these principles/laws explains why phenotypic and genotypic ratios can be different. a. Independent Assortment....
Which of these principles/laws explains why phenotypic and genotypic ratios can be different. a. Independent Assortment. b. Uniformity c. Dominance d. Equal segregation
which of following pricing methodologies best describes a methodology in which the transfer pricing is computed...
which of following pricing methodologies best describes a methodology in which the transfer pricing is computed by adding an appropriate profit markup to the sellers total cost of a product
In 300 words or more answer the following questons: 1. Which System Development Methodologies (SDLC, Prototyping,...
In 300 words or more answer the following questons: 1. Which System Development Methodologies (SDLC, Prototyping, RAD, JAD, or Object Oriented) is appropriate to be used for what type of Systems? Hint: Look at characteristics of different systems. 2. What is "Total Cost of Ownership (TCO)" and which of the methodology in question 1 has a better TCO?
Which statement is true according to the American Nurse associates ANA Handle with care fact sheet?
Which statement is true according to the American Nurse associates ANA Handle with care fact sheet?
Which of the transfer pricing methodologies do you find the most compelling and why? What factors...
Which of the transfer pricing methodologies do you find the most compelling and why? What factors influenced your decision?
Why are accounting principles different for Municipal Solid Waste Landfills
Why are accounting principles different for Municipal Solid Waste Landfills
Taxation of Executive Compensation . Explain the different tax principles applied to deferred and current compensation...
Taxation of Executive Compensation . Explain the different tax principles applied to deferred and current compensation plans?
Which of the four collaborative conflict-management principles do you most frequently use? Which of the principles...
Which of the four collaborative conflict-management principles do you most frequently use? Which of the principles do you least frequently use? Describe a situation in which you were dealing with a difficult group member and it didn’t go as well as you hoped. Describe a strategy why it may have been more effective?