Question

true or false

1. A -static agent inhibits the growth of an organism perpetually

2. "To determine the minimum bactericidal concentration of a compound against an organism, the contents of the MIC test tubes must be inoculated to new broth media. "

3. The concentration of antimicrobial drug in the disk must be consistent in the Kirby-Bauer test.

Identification

1. An antibiotic that acts on both Gram-positive and -negative bacteria is called a ___ antibiotic.

2. An antibiotic that has a chemical structure based on a naturally-occuring antibiotic but has already undergone partial modifications is called a ___ antibiotic.

3. How many Mueller-Hinton agar plates will be used in a Paper Disk Diffusion assay where there should be triplicate data for 2 test organisms and 3 concentrations of 2 antibiotics each.

Answer 1

1. True

Explanation : A static agent prevents the organism from multiplying and thus helps the human immune system in killing it and leading to recovery from the infection

2. True

Explanation : Minimum bactericidal concentration can be determined from broth dilution of MIC tests by subculturing to agar plates that do not contain the test agent.

3. False

Reason : The concentration for antimicrobials like Ampicillin and Cefazolin are different on the disk. Ampicillin(16-22mm) and Cefazolin(21-27mm)