Question

sketch out how you will do the glucose oxidase test. how many vials, kind of samples and how much what goes in each tube.

Answer 1

In the glucose oxidase assay, the
glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen peroxide. The hydrogen peroxide is then oxidatively coupled with a chromogen to produce a colored compound which may be measured spectroscopically.
Glucose oxidase catalyse the oxidation of Beta D-glucose present in the plasma to D glucono -1 ,5 - lactone with the formation of hydrogen peroxide; the lactone is then slowly hydrolysed to D-gluconic acid. The hydrogen peroxide produced is then broken down to oxygen and water .The acidification of blood samples using a combination of citrate buffer, NaF, and EDTA was more effective.