Question

	Amount in test tube	Absorbance	Concentration
1: 100 solution	20 microliters	.212A	.12A
1:1000 solution	20 microliters	.344A	.16A

what is the total amount of protein in each fraction? when the inital solution before dilution was .5ml and the standard curve equation is y=.44x, R^2=.08

Answer 1

Since we have been given the standard curve equation, we will be using it to create the Beer Lambert's equation using analogy as shown below:

A = 0.44*C

Here, C is the conc of the analyte solution in mol/L

For first fraction,

A = 0.212

So,

C = 0.212/0.44 = 0.482 M

Since this is a 1:100 solution, this means that it is diluted 100 times.

So, original solution conc. = 48.2 M

Similarly for second fraction we have:

A = 0.344

So,

C = 0.344/0.44 = 0.782 M

Since this is a 1:1000 solution, this means that it is diluted 100 times.

So, original solution conc. = 782 M