Question

what are 2 ways to achieve a better resolution of two proteins on a size exclusion chromatography column?

What are 2 reasons for protein recovery loss from a size exclusion chromatogrpahy column?

Answer 1

Size exclusion chromatography media with high uniformity and narrow particle size distribution facilitate the elution of molecules in sharp peaks. Size exclusion chromatography media with smaller particle sizes facilitate diffusion of sample molecules in and out of the particles by reducing the time to achieve equilibrium between mobile and stationary phases and so improve resolution by reducing peak width.

Protein is unstable or inactive in the buffer.

. Protein has been degraded by proteases.