Question

The following is a hypotheticalgene that codes for a wonderful peptide in your body that magically dissolves extra fat that you may consumme. Assume it is the template strand. What is the primary structure of the peptides? (Use three letter abbreviations) Draw the peptide at a pH of 7, 1 and 13. Indicate what the overall charge would be at these three pH's.

5' CTCGGGACCAGGGGTTAAAGTCGTAGTTTTTCCCCGTCGGCTTAGT 3'

(The bold faced areas are introns)

Answer 1

Yours strand: 5' CTCGGGACCAGGGGTTAAAGTCGTAGTTTTTCCCCGTCGGCTTAGT 3'

First let´s define that yor the introns are not Transcripted, so: 5'CTCGGGACCAAAGTCGTAGGTCGGCTTAGT 3'

Then, we do the Codified Codon(C=G; G=C; T=A; A=U)

In the process of transcripion the sense is inverted so our ARNm is:

3´GAGCCCUGGUUUCAGCAUCCAGCCGAAUCA5´

For The codon Read the sense of reading is 5´-3´so for the triplets we have to invert our chain

5´ACUAAGCCGACCUACGACUUUGGUCCCGAG3´

Now our triplets=peptide using the next table:

ACU=Thr

AAG= Lys

CCG= Pro

ACC= Thr

UAC= Tyr

GAC= Asp

UUU= Phe

GGU= Gly

CCC= Pro

GAG= Glu

And our Primary structure is:

N-Thr Lys Pro Thr Tyr Asp Phe Gly Pro Glu-C

The draw part you have to post in another question because is a different process.