Question

In the quantitative analysis for this experiment, you have to calculate a "relative response factor" for...

In the quantitative analysis for this experiment, you have to calculate a "relative response factor" for each analyte in order to determine the relative amount of each analyte in the mixture. Briefly explain why you have to do this and you can't just use the absolute integrated area under each of the analyte peaks in the chromatograph to determine relative amounts.

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Answer #1

Quantitative analysis

From the standard solution, prepare set of dilute solutions of known concentration

Inject and record the area of each concentration.

Plot, area on y-axis vs concentration on x-axis (calibration plot)

Now for the unknown, inject and record the area under this peak

from the calibration plot we can determine concentration of the unknown.

response factor = (area for unknown/concentration of unknown)/(area for standard/concentration of standard)

So we have all four terms known in the formula, we can easily determine response factor for the compound.

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