Question

2. An unknown protein yields the following test results using the procedure in this experiment. based...

2. An unknown protein yields the following test results using the procedure in this experiment. based on these results, what amino acids must be present in the protein?

Test: Observation:

Ninhydrin Yellow Solution

Xanthoproteic (+) yellow solution

Millon's (+) red precipitate

Hopkins-Cole (-) colorless

Nitroprusside (+) purple solution

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Answer #1

Ninhydrins test give yellow color detecting the presence of Proline and Hydroxy proline in the protein.

Xanthoprotic test give positive test and yellow color detecting the presence of Tyrosine and Tryptophan in the protein.

Millon's test give positive test and red color detecting the presence of Tyrosine in the protein.

Hopkins cole test give negative test (colorless) detecting the absence of Tryptophan in the protein.

Nitroprusside test give positive test and purple color detecting the presence of Cysteine in the protein.

Therefore the aminoacids that must be present in the Protein are Proline, Hydroxy proline, Tyrosine and Cysteine

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