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What is the order of the chymotrypsin catalyzed reaction at high substrate concentrations? Can someone tell...

What is the order of the chymotrypsin catalyzed reaction at high substrate concentrations? Can someone tell me and explain why is that?

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Answer #1

According to Micheal Menton equation for enzyme catalysis,

Rate of enzyme catalysis,R = K2 [E°][S°]/Km+[S°]

where K2 is turnover number

Km is micheal menton constant

[E°] is initial enzyme concentration

[S°] is intial substrate concentration

Now at high substrate concentration for chymotrypsin enzyme catalysis, [S°] >>>Km

So Km can be neglected in denominator, so that

Rate , R =K2 [E°][S°]/[S°]

R= K2 [E°]

This implies that rate is ZERO ORDER with respect to substrate concentration at high substrate concentration.

  

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