Question

(a) Calculate the PI of the peptide below: Met-His-Leu-Pro-Asn-Glu b) Which method would you use to...

(a) Calculate the PI of the peptide below: Met-His-Leu-Pro-Asn-Glu b) Which method would you use to purify the peptide in question 1: Gel filtration chromatography, or ion exchange chromatography? Why? What resin would you use?

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What would be the charge of this peptide, Asn-Arg-Met-Lys-His-Pro-Trp-His-Thr-Tyr-Ser-Gly-Glu-Leu-Val-Trp, at a pH of 1 and a...
What would be the charge of this peptide, Asn-Arg-Met-Lys-His-Pro-Trp-His-Thr-Tyr-Ser-Gly-Glu-Leu-Val-Trp, at a pH of 1 and a pH of 11?
Write down the mRNA sequence for: met-ser-leu-gly-trp-stop met-ser-pro-cys-his-stop met-ile-asn-ala-tyr-stop met-gln-thr-leu-arg-cys-pro-lys-stop start-ile-glu-asp-ser-his-trp-stop start-leu-pro-gln-tyr-ile-met-val-ala-stop start-ala-glu-trp-ser-phe-pro-
Write down the mRNA sequence for: met-ser-leu-gly-trp-stop met-ser-pro-cys-his-stop met-ile-asn-ala-tyr-stop met-gln-thr-leu-arg-cys-pro-lys-stop start-ile-glu-asp-ser-his-trp-stop start-leu-pro-gln-tyr-ile-met-val-ala-stop start-ala-glu-trp-ser-phe-pro-ser-gly-thr-stop start-val-ala-thr-thr-leu-tyr-cys-gly-arg-stop start-lys-asn-gly-phe-his-thr-arg-pro-gln-stop start-met-thr-asn-lys-pro-gln-ser-leu-arg-stop
Calculate the net charge on the following tetrapeptides. Asp-Arg-Ser-Pro Trp-Gln-Ile-Lys Cys-Asn-Glu-Tyr Which peptide will be retained...
Calculate the net charge on the following tetrapeptides. Asp-Arg-Ser-Pro Trp-Gln-Ile-Lys Cys-Asn-Glu-Tyr Which peptide will be retained the longest on a cation-exchange column at pH 7?
Some characteristics of three proteins are listed in the table below: Protein Molecular Weight (Da) Isoelectric...
Some characteristics of three proteins are listed in the table below: Protein Molecular Weight (Da) Isoelectric point (pI) Does the Protein Contain a heme moiety? 1 65,000 5.5 No 2 54,000 10.0 No 3 14,500 4.5 Yes a.(2 points) What type of chromatography separates proteins based on size? b. (2 points) What type of chromatography separates proteins based on their charge? c.(10 points) Could gel filtration chromatography be used to separate a mixture containing Protein 2 and 3? Clearly explain...
you have following proteins protein PI MM Specific tag 1 6.4 28700 his6 tag 2 4.5...
you have following proteins protein PI MM Specific tag 1 6.4 28700 his6 tag 2 4.5 33100 no tag 3 6.5 210111 HIS6 TAG Which of the following methods would be the best method for separation? 2d gel electrophoresis, ion exchange size exclusion isoeletric focusing native page SDS page affinity chromatography 2D page
Which of these best explain why you would calculate a company's cost of capital and use...
Which of these best explain why you would calculate a company's cost of capital and use it as the discount rate for evaluating a potential investment?                 a.This approach best reflects the risk profile of the company                 b.It is required by the Securities and Exchange Commission                 c.It is fun to write Excel formulas to calculate it The price of ABC Corp. stock was $100 per share on Jan. 1, 2018 and $110 on Dec. 31, 2018. The total...
For this assignment you must use your knowledge of the scientific method to design your own...
For this assignment you must use your knowledge of the scientific method to design your own experiment. The first step of the scientific method is observation. Answer the questions below about how you would design your scientific experiment. Lab 1: Using The Scientific Method Worksheet At A Local Town Meeting, One Of Your Neighbors ... Question: Lab 1: Using the Scientific Method Worksheet At a local town meeting, one of your neighbors compl... Lab 1: Using the Scientific Method Worksheet:...
Question 3 You work in a manufacturing company which produces white goods. The company is planning...
Question 3 You work in a manufacturing company which produces white goods. The company is planning an investment in a new product line. The initial investment includes the machinery required for the production line which costs $320,000. Other expenses were included in the expected cash flows. The new machinery has a useful life of 4 years and an estimated residual value at the end of the 4th year of $80,000. Depreciation is the only non-cash expense. The net cash flows...
The picture shown below shows variation among three individuals with respect to 4 nucleotides - AGAT....
The picture shown below shows variation among three individuals with respect to 4 nucleotides - AGAT. What do you think what type of variation is this? AGAT different repeat numbers in different individuals .png Minisatellite Single nucleotide polymorphism Short Tandem Repeats Which of the following statements is TRUE about DNA matching? Typical difference between the genomes of human beings and Chimpanzees is estimated to be 25 % Typical difference between the genomes of human beings and Drosophila is   estimated to...
Question 1 [10 marks] Houston House (Pty) Ltd and Whitney Holdings are competitors in the same...
Question 1 [10 marks] Houston House (Pty) Ltd and Whitney Holdings are competitors in the same industry. The following information was summarised from a recent annual report of Houston House (Pty) Ltd (In millions) Receivables: December 31, 2015 R 1,968 December 31, 2014 642 Revenue for the year ended: December 31, 2015 46,980 December 31, 2014 40,023 The following information was summarised from a recent annual report of Whitney Holdings: (In millions) Accounts and notes receivable, net December 31, 2015...