Question

I am a cancer researcher. I have an antibody that recognizes the extracellular portion of Notch and I have conjugated it to a light producing chromophore. I know activated Notch is implicated in many types of tumors. I want to detect for a given tumor whether Notch activity has changed in comparison to wild type cells of the same type. Based upon what you know, which of the following statements could be true? (Hint: Use the information from the other qustions.)

	A)	I need to separate out proteins based upon charge.
	B)	I could perform PAGE for analysis.
	C)	I could perform agarose gel electrophoresis for analysis.
	D)	Tumor cells where Notch is activated should contain 3 Notch protein that I can see using my antibody.
	E)	Tumor cells where Notch is activated should contain 2 Notch protein that I can see using my antibody.
	F)	I need to use SDS and beta-mercaptoethanol.
	G)	Tumor cells where Notch is activated should contain 1 Notch protein that I can see using my antibody.
	H)	I should blot the gel.
	I)	I could make a 2D gel for analysis.
	J)	I need to separate out proteins based upon size.
	K)	I must use my antibody to see the Notch protein.

Answer 1

The things you can do to sort out this problem is:

1) you should blot the gel

2) you can also separate the protein on the basis of the size.

3) you can also observe it through the light detection given by the fluorophore.

4) the notch will activate two notch protein on the surface.

You must use the antibody to see the notch protein... tumour cells where the notch is activate should contain 1 notch protein that u can see using your antibody...then you should detect the protein-antibody complex based on their size.