Question

What similarities are there between GC, column, and TLC chromatography

What similarities are there between GC, column, and TLC chromatography

Homework Answers

Answer #1

Gas Chromatography (GC), Column Chromatography and Thin Layer Chromatography (TLC) all these chromatographic protocols use the basic principle of Chromatographic separation. The similarities are listed as following:

  1. Chromatography is based on the principle of separation of compounds into different bands and then identification of those bands.
  2. The preferential separation is done due to differential affinities of compounds towards stationary and mobile phase. After separation of the compounds they are identified by suitable detection methods.
  3. The differences in affinities arises due to relative adsorption or partition coefficient in between components towards the both phases.
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