Question

Is the GFP-Ras protein present in the the golgi or nucleus? How does one know using Fluorescence Spectroscopy?

Answer 1

GFR (Green fluorescent protein)-Ras protein present in the Golgi and one can easily know these GFP-Ras protein using Fluorescence Spectroscopy as the GFR -like protein is a rapid growing family which contain 200 fluorescent protein and emits fluorescent and as in Fluorescence Spectroscopy the analyte concentration is directly proportional to the intensity of emission and in this case with the GFR-Ras protein emits fluorescent which is directly proportional to the concentration.