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For the Biuret, Lowry, Bradford, and BCA Assay: Why might you expect the different methods for...

For the Biuret, Lowry, Bradford, and BCA Assay:

Why might you expect the different methods for determination of protein concentration to give different results?

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The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, is a biochemical assay for determining the total concentration of proteinin a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

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