Question

Fluorescence spectroscopy relies on:

A. the difernce in color between two samples

B. the shift in wavelength from excitation and emossion

C. the vibrations of atoms bonded together in a molecule

D. the atomic mass of the sample

E. the formation from specific ions

Answer 1

The basic of fluorescence-

Electrons of a molecule jumps from the singlet ground state to an excited state when absorb light (photons). Electrons will return to the ground state from an excited state following two consecutive steps:

(i) The electron loses part of its energy to the surrounding environment to come down to ground state which is called the inter system crossing.

(ii) Another pathway is via competitive processes: emission of a photon (fluorescence) with a radiative rate constant kr

So, the answer is B. the shift in wavelength from excitation and emission