Question

1. Mention the most important difference or similarities between the following.

a.) Stationary Phase in column chromatography and Mobile phase in column chromatography.

b.) Gravity Column chromatography and Flash Column chromatography

c.) Thin Layer chromatography and column chromatography.

Answer 1

a.) Stationary Phase in column chromatography and Mobile phase in column chromatography.

similarities --> they are used to retain the solvents, either polar or nonpolar.

differences --> the stationary phase does not moves, and typically is polar (silica) for the mobile phase, we require a nonpolar solvent , it acutally moves across the TLC plate

b.) Gravity Column chromatography and Flash Column chromatography

gravity column --> requires solvent which move with coheisve forces

flash column --> uses vaolatile flashing properties (evaporation) of the solvents

c.) Thin Layer chromatography and column chromatography.

both applies the concept of stationary phase in column style

difference --> thin layer chromatography uses veyr thin plates, whereas the column chromatography uses a physcal colunn as stationary phase