Jasper incubated mitochondria in labeled water H2O18 and analyzed all the intermediates of the citric acid cycle to find where O18 was incorporated. He found O18 was incorporated twice. Explain exactly which enzyme incorporated the O18 and where in the structure it was located.
Aconitase & Fumarase both enzymes incorporated the labelled O18 atoms as shown in the below image (Magenta circled ones).
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