Question

In each dilution step, we will pipet a certain volume (calculate and insert it to teh table using prelab preparation) of the 2.5 mM APAP standard solution into 50 mL volumetric flask, and dilute to volume. Calculate the exact concentration for each solution.

Start with	Deliver (mL Flask)	Dilute to volume with pH 2.2 Mellvaine buffer and Obtain
25 mM APAP stock	? mL into 50	2.5 mM
2.5 mM	? mL into 50	0.5 mM
0.5 mM	? mL into 50	0.05 mM
25 mM APAP stock	? mL into 50	1.0 mM
1.0 mM	? mL into 50	0.2 mM

Answer 1

Unknown volume of Stock solution of given molarity is taken in 50 ml volumetric flask to prepare a standard solution of given concentration by dilution .

For dilution, M1*V1 = M2*V2

where, M1 = molarity of stock solution

V1 = volume of stock solution

M2 = molarity of standard solution prepared

V2 = volume of standard solution prepared

1.

25 mM * V1 = 2.5 mM * 50 mL

V1 = 5 mL

2.

2.5 mM * V1 = 0.5 mM * 50 mL

V1 = 10 mL

3.

0.5 mM * V1 = 0.05mM * 50 mL

V1 = 5 mL

4.

25mM * V1 = 1.0 mM * 50 mL

V1 = 2 mL

5.

1.0 mM * V1 = 0.2 mM * 50 mL

V1 = 10 mL