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What fraction of enzyme molecules is bound to substrate when the substrate concentration equals Km?

What fraction of enzyme molecules is bound to substrate when the substrate concentration equals Km?

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Answer #1

Michaelis–Menten equation is used typically in enzymatic reactions.

V = Vmax*[S] / (Km + [S])

Where

Vmax = max rate velocity

[S] = substrate concentration

Km = Michaelis–Menten constant

V = reaction rate


Michaelis–Menten equation is used typically in enzymatic reactions.

V = Vmax*[S] / (Km + [S])

Where

Vmax = max rate velocity

[S] = substrate concentration

Km = Michaelis–Menten constant

V = reaction rate

then...

if [S] = Km

then

V = Vmax*[S] / (Km + [S])

V = Vmax*[S] / ([S]+ [S])

V = Vmax * 1/(2)

V = 1/2*Vmax

the fraction is 0.50

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