Question

A biochemist obtains the following set of data for a mouse enzyme that is known to follow Michaelis- Menten kinetics. The experimental conditions were with 0.1 μmol of enzyme and pH 6.0.

Substrate Initial concentration velocity (μM) (μmol/min) and Initial velocity

1 49

2 96

8 349

50 621

100 676

1,000 698

5,000 699

Question 1. Estimate what would be the initial velocity in μmol/min at a substrate concentration of 10,000 μM.

Question 2. What is the approximate Vmax for this enzyme in μmol/min? Do not put units in blackboard.

Question 3. Explain how you came up with the answers to question 1 and 2

Question 4.What is the approximate km of this enzyme in uM

Question 5.Explain how you came up with the answer to Question 3

Question 6. What is the approximate Kcat for this enzyme?

Question 7.Explain how you came up with answer to question 6

Question 8.The prososed catalyic reaction requires a depronated His -R group. true or false, increasing the pH to 7.0 would you expected the increase in Vmax
increasing the pH to 7.0 would be expected to increase the Vmax.

Question 9. Explain your answer to question 8.

Question 10. 0.01 μM of the compound salicylic acid was added to all samples in the experiment above. At substrate concentrations less than 50 μM the initial rate of the enzyme was greatly reduced, however at concentrations greater than 1,000 μM there was little effect on the initial rate. What is the name of the phenomenon cause by salicylic acid?

Please help Thanks!!!!!

Answer 1