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Calculate the volume (in mL) of a 0.220 M KOH solution that should be added to...

Calculate the volume (in mL) of a 0.220 M KOH solution that should be added to 4.750 g of HEPES (MW=238.306 g/mol, pKa = 7.56) to give a pH of 7.60. Give answe in mL.

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Answer #1

moles of HEPES = 4.750 / 238.06 = 0.01995

moles of KOH = 0.220 x V

HEPES      + KOH   ---------------> salt   + H2O

0.01995    0.220V                        0          0

0.01995-0.220V        0                             0.220V

pH = pKa + log [salt / acid]

7.60 = 7.56 + log [0.220 V /0.01995-0.220V ]

[0.220 V /0.01995-0.220V ] = 1.096

V = 0.474 L

volume of KOH = 474 mL

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