Thermodynamic constrains. Compare the delta G' standard values for the oxidation of succinate by NAD+ and by FAD. Use the data given to find E'0 of the NAD+-NADH and fumerate-succinate couples, and assume that E0' for the FAD-FADH2 redox couple is nearly 0.05 V. Why is FAD rather than NAD+ the electron acceptor in the reaction catalyzed by succinate dehydrogenase?
Given
E0' NAD NADH -0.32 V
E0' fumarate - succinate +0.03
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