Question

The amount of calcium in physiological fluids can be determined by a complexometric titration with EDTA....

The amount of calcium in physiological fluids can be determined by a complexometric titration with EDTA. In one such analysis a 0.100-mL sample of a blood serum was made basic by adding 2 drops of NaOH and titrated with 0.00119 M EDTA, requiring 0.268 mL to reach the end point. Report the concentration of calcium in the sample as milligrams Ca per 100 mL.

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Answer #1

hello!.I have done the calculution based on the assumption that the Ca sample is prepresd in a 100ml flask.

Mllimoles od EDTA titrated =Total Millimloes of Ca sample

Millimoles of EDTA=mL of EDTAxMolarity of EDTA=0.268x0.00119=0.000318

total Millimoles of Ca=millimoles of Cax(100/0.100)mL=0.000318

Millimoles of Ca =0.000318/1000=0.000000318

Milllimoles of Ca in the aliquot=0.000000318x40.078g/mol=0.0000127 (M1V1=M2V2)

mass of Ca=0.0000127x(100/0.1)mL=0.0127mg

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