Question

What are some of advantages and disadvantages of fluorescent spectroscopy microscopy techniques over optical microscopes?

Answer 1

1. the method produces a very good dark field image that improves contrast

2. any objective lens can be used as long as its NA is less than the condenser’s, even for ultra violet (UV) excitation

3. this method produces very bright images at low magnification.

Optical Microscopy having following disadvanyages

1. Magnification. At a cap of roughly 2,000 times

2. Resolution.  lower resolution.

3. Observing Internal Structures. The internal structures of living things are difficult to observe without the use of dyes.