Question

What determines the electrophoretic mobility of an analyte when it migrates in a capillary electrophoresis (CE) column?

Answer 1

Electrophoresis is a class of separation techniques in which we separate analytes by their ability to move through a conductive medium which is usually an aqueous buffer in response to an applied electric field.

Cations with larger charge-to-size ratios,which favors ions of larger charge and of smaller size which migrate at a faster rate than larger cations with smaller charges.

Anions migrate toward the positively charged anode and neutral species do not experience the electrical field and remain stationary.