Why is it a good idea to deoxygenate analyte solutions for fluorometric determinations?
Let us understand the basics of flourometric titration first
It is a type of e.m. spectroscopy that analyzes fluorescence from a sample. It involves using a beam of ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy. In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores.
Thereare several reasons behind deoxygenation which are as follows
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