Question

What other class of molecule besides salts are commonly used for fractional precipitation of proteins

What other class of molecule besides salts are commonly used for fractional precipitation of proteins

Homework Answers

Answer #1

Following molecules can also be used to precipitate proteins in addition to salts :

1. Miscible solvents, like Ethanol and Methanol : They replace water layer from the protein and cause aggregation of proteins.

2. Polymers, for example Dextran and Polyethylene Glycol : These polymers also attract water molecules away from proteins and cause it to precipitate.

3. Alginate, tannic acid etc can also be used.

4. Metal ions can also be used , for example : Ca2+, Mg2+ etc.

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