Question

2) Buffers are extremely important in biology as they allow you to prepare solutions with a...

2) Buffers are extremely important in biology as they allow you to prepare solutions with a specific pH that can match physiological conditions. To prepare a buffer, we frequently take a weak acid and react it with a strong base.

2a) In this reaction, we combine 1 ml of 0.1 M NaOH with 10 ml 1M CH3CO2H (acetic acid). Write the reaction that proceeds (it goes to completion) and determine the amount of each component.

2b) Using the Henderson-Hasslebach equation we can determine the pH of the resulting solution. The Ka of Acetic Acid is 1.8 ∗ 10−5

henderson hasslebach equation: pH = pKa + log10([A-]/[HA])

2c) Is the resulting solution acidic or basic?

Homework Answers

Answer #1

the reaction requires more then or equal 10 ml of NaOH to proceed

the amount of NaOH required (1ml) is 4 mg (for preparation of 1000 ml 0.1 M NaOH solution we need 4 gm or 4000 mg)

the amount of Acetic acid required (10 ml) is 605 mg ( for preparation of 1000 ml 1M Acetic acid solution 60.50 gm)

from the Henderson-Hasslebach equation the pH is calculated as follows

pH = pKa + log (CH3COO-)/(Na+)

= -log1.8 x 10-5 + log (0.605)/(0.004)

= 3.744 + log (151.25)

= 3.744 + 2.178

pH = 5.923

So the resulting solution is acid in nature

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