Question

Explain how natural chromophores can be used to calculate concentration of proteins. Calculate the extinction coefficient of bovine chymotrypsin (1YPH). Show your work.

Answer 1

natural chromophores are nothing but light absorbing groups. This molecular moieties likely to absorb light in the 200 to 800 nm region are pi-electron functions and hetero atoms having non-bonding valence-shell electron pairs.The presence of chromophores in a molecule is best documented by UV-Visible spectroscopy,from the below equation we can calculate the concentration of protein using its absorbance, molar absorptivity,and length of light path through the sample in cm.Extinction coefficient: E1%= 20.4 (280nm)arbitary value

Molar Absorptivity, ? = A / c l