Question

This question is regarding bradford and ninhydrin assays. What is the concentration range over which each...

This question is regarding bradford and ninhydrin assays.

What is the concentration range over which each amino acid is linear?

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Compare the Bradford assay and the Bicinchonic Acid Assay. Which is likely to give the most...
Compare the Bradford assay and the Bicinchonic Acid Assay. Which is likely to give the most accurate estimate of protein concentration. Explain why A280can be used to measure protein concentration. Is this method better suited to complex samples or purified proteins? After preparing a standard curve of diluted BSA protein in a BCA assay you get a trendline with the equation A562 = 0.0011[BSA] (mg/mL) + 0.0378. Rearrange the equation to solve for [BSA] (mg/mL). If a 1:8 dilution of...
I am asked to prepare a bradford assay in class using gamma-globulin (to determine the concentration...
I am asked to prepare a bradford assay in class using gamma-globulin (to determine the concentration of protein (a-La in original milk and multiple other samples) in order to do this I must prepare a strand curve (calibration curve) with samples containing known concentrations of gamma-globulin. The dilutions are made with a buffer A used in lab and the final volume of each dilution must be at least 200 uL. The standard curve will range from 0 to 60 mg/mL...
Long Answer 1. You perform a Nirenberg and Ochoa type experiment in which synthetic RNAs are...
Long Answer 1. You perform a Nirenberg and Ochoa type experiment in which synthetic RNAs are made by incubating polynucleotide polymerase with CDP and UDP (80% CDP to 20% UDP ratio). What ratios of each of the eight possible codons do you expect? 2. What percentages of radioactivity would you expect for each of the 20 amino acids when a cell free translation assay is run 20 times in which each time a different radioactive amino acid is included with...
question 1: For a strong acid with an initial solution concentration of 0.065 M, what do...
question 1: For a strong acid with an initial solution concentration of 0.065 M, what do you think will be the approximate concentration of acid molecules, the hydronium ions, and the conjugate base ions? Question 2: For a weak acid, why is it not possible to make the same assumptions about the final product and reactant concentrations of a strong acid?
What is the open circuit potential (or the range over which OCP must exist) for the...
What is the open circuit potential (or the range over which OCP must exist) for the following electrochemical cells? Is the cell galvanic or electrolytic as written? If spontaneous, what two half reactions will occur? Glassy carbon electrode (GCE) / Fe(CN)64- (10 mM), KCl (0.5 M) // KCl (sat'd) // H2SO4 (0.1 M) / Pt
1. Which of the following amino acids is properly paired with a property? Question options: a)...
1. Which of the following amino acids is properly paired with a property? Question options: a) glycine: contains a polar R group b) proline: presence in polypeptide may cause abrupt change in direction and/or restricted geometry c) leucine: basic amino acid d) aspartate: generally positively charged at neutral pH e) valine: an amino acid that is ideal for the transfer of protons within the catalytic site of enzymes due to the presence of significant amounts of both the protonated and...
Explain the circumstances for which the interquartile range is the preferred measure of dispersion. What is...
Explain the circumstances for which the interquartile range is the preferred measure of dispersion. What is the advantage that the standard deviation has over the interquartile range?
Please answer the following: Are the following answers correct? 1. Which is NOT ture regarding 2,3-bisphosphoglycerate?...
Please answer the following: Are the following answers correct? 1. Which is NOT ture regarding 2,3-bisphosphoglycerate? a. It binds to oxygen <-- FALSE b. It binds in the central cavity of hemoglobin c. It prevents oxygen binding d. It stabilizies deoxyg hemogloblin 2. The Fe binds all of the following except ______________ a. Proximal His b. Several N of the Heme group c. Distal His d. Oxygen <-- CORRECT 3. The shape of hemoglobin binding oxygen is ________ while that...
When 50.00 mL of an acid with a concentration of 0.122 M (for which pKa =...
When 50.00 mL of an acid with a concentration of 0.122 M (for which pKa = 4.94) is titrated with 0.100 M NaOH, what is the pH at the equivalence point? Which one of the following indicators could be used for the titration? The approximate pH range of the color change has been included in parenthesis. Methyl green (0.2-1.8) Ethyl red (4.0-5.8) Phenolphthalein (8.2-10.0) or None of these
What is the hydronium ion concentration of a 1.98x10^-4 M  solution of p-bromobenzoic acid, , for which...
What is the hydronium ion concentration of a 1.98x10^-4 M  solution of p-bromobenzoic acid, , for which Ka= 1.00x10^-4 ?
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT