Question

The protein apotransferrin binds two Fe(III) ions for cell transport. Consider when a 2.50 mL solution of apotransferrin is titrated with 1.10 � 102 ?L of a 1.26 M Fe(III) solution. The initially colorless solution turns red, and the color change can be measured with a spectrophotometer. First, explain why there is a difference in spectrophotometric response during titration before and after the equivalence point by placing the statements below into the appropriate categories.

Now, calculate the concentration of apotransferrin present in the original 2.50 mL solution.

Answer 1