Question

Volumetric Pipet Procedure:

Order the steps for the procedure for using the volumetric pipet to deliver a fixed volume of water. 1-First Step, 8-Last Step.

( )Before starting make sure that the pipet is clean and is not chipped.

( )When done, rinse pipet with tap water and then distilled water before storing.

( )Now fill the pipet with liquid to above the calibration mark or line with suction from bulb -- without getting liquid in the bulb.

( )Drain freely into the receiver container, wait 5 seconds after flow stops and touch off last drop to transfer all of the calibrated volume.

( )Before starting the transfer rinse the pipet by partially filling it with liquid to be delivered-- do not get liquid in bulb.

( )Wipe the excess liquid off the lower stem and tip of pipet. (This step before the final adjustment of the meniscus level to the mark).   

( )Remove bulb and immediately cover the top of the pipet stem with your index finger.

( )Adjust level so the bottom of the meniscus is on the calibration mark by slow release of pressure.

Answer 1

( 1) Before starting make sure that the pipet is clean and is not chipped.

(8 )When done, rinse pipet with tap water and then distilled water before storing.

( 3)Now fill the pipet with liquid to above the calibration mark or line with suction from bulb -- without getting liquid in the bulb.

(7 )Drain freely into the receiver container, wait 5 seconds after flow stops and touch off last drop to transfer all of the calibrated volume.

( 2)Before starting the transfer rinse the pipet by partially filling it with liquid to be delivered-- do not get liquid in bulb.

(5 )Wipe the excess liquid off the lower stem and tip of pipet. (This step before the final adjustment of the meniscus level to the mark).   

( 4)Remove bulb and immediately cover the top of the pipet stem with your index finger.

( 6)Adjust level so the bottom of the meniscus is on the calibration mark by slow release of pressure.