Question

What is the transition state stabilization or binding energy? Why does an enzyme destablize the interaction between the active site and the substrate? How does it do this?

Answer 1

We know that enzymes acts like a catalyst to speed up the reaction and in order to do this they
stabilize the transistion state by minimizing its energy. This is called transistion state stabilization.
Binding energy is the energy released when a protein or an enzyme binds to the substrate.

The active site structure is modified in such a way that it fits the transition state better than the
substrate and when this happens the enzyme may destabilize a bond and weaken it so that less
activation energy is required to break that particular bond.

For an enzyme to destablize the interaction between the active site and the substrate
(i) It can induce some kind of distortion or strain in the molecule
(ii) Minimizing the binding capacity.
(iii)Desolvation