Question

In this experiment, we are dong quantitative determination of Caffeine by reversed-phase high pressure liquid chromatography...

In this experiment, we are dong quantitative determination of Caffeine by reversed-phase high pressure liquid chromatography (HPLC).

This experiment was carried out under isocratic conditions. What are the advantages of gradient elution?

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Reversed Phase gradient HPLC is characterised by the analyte behaviour under the influence of the gradient...
Reversed Phase gradient HPLC is characterised by the analyte behaviour under the influence of the gradient conditions. Which of the following statements are correct (may be more than one correct statement): a. Retention factors are calculated in the same way as in conventional isocratic HPLC b. Most analyte molecules will remain wholly partitioned in the stationary phase at the beginning of the gradient analysis. c. The analyte hydrophobicity will dictate its rate of acceleration through the column d. The retention...
In this lab, we are trying to determine the concentration of caffeine in caffeinated beverages using...
In this lab, we are trying to determine the concentration of caffeine in caffeinated beverages using high pressure-liquid chromatography (HPLC) and UV absorbance detector. Why is it important that the pressure remain constant during an HPLC run?
In this lab, we are trying to determine the concentration of caffeine in caffeinated beverages using...
In this lab, we are trying to determine the concentration of caffeine in caffeinated beverages using high pressure-liquid chromatography (HPLC) and UV absorbance detector. Why was a dector wavelength of 284 nm chosen? How would changing the dector wavelength to 190 nm impact the sensitivity of the method for caffeine?
Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography (RPLC). On...
Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography (RPLC). On the first run, using a mobile phase containing 40% tetrahydrofuran and 60% water, the peaks all elute between 0 and 3 min and are too close together to be quantitatively resolved. To improve the resolution of the peaks, should you increase or decrease the amount of water in the mobile phase for the next run? Or is there not enough information? In a separate...
1. High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical...
1. High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 2.40×108 Pa , what is its running pressure in torr? Express the pressure numerically in torr. 2.40×108 Pa = ______ torr 2. A sample...
Why didn't we use ethanol instead of dichloromethane to extract caffeine from the energy drink? Sublimation...
Why didn't we use ethanol instead of dichloromethane to extract caffeine from the energy drink? Sublimation can be an excellent technique for purifying a compound with which of the following specific characteristics? A High vapor pressure at a temperature above its melting point B High vapor pressure at a temperature below its melting point C Low vapor pressure at a temperature above its melting point D Low vapor pressure at a temperature below its melting point How is it possible...
1-The temperature value inside a reactor in the treatment plant is 30 0C during operation. The...
1-The temperature value inside a reactor in the treatment plant is 30 0C during operation. The outdoor temperature in the winter period is 5oC. The wall thickness of the reactor is 0.10 m and its area is 20 m2. Air and reactor wall heat transfer coefficients and conductivity are given below. reactor = 20W / m2.K, creator = 0.8 W / m.K; outdoor environment = 10 W / m2.K a. Under these conditions, calculate the heat loss value from the...
I have provided the information for the lab below. I want to see the chemical reactions...
I have provided the information for the lab below. I want to see the chemical reactions that take place during each step of the experiment. So in the procedures when a chemical was added i would like to see what the products and byproducts that were formed. I also want to see one over all reaction for this experiment to just get an overview of what exactly is happening during the experiment. Thank you:) Part B: Synthesis of [Co(NH3)5Cl]Cl2 Objective:...
What tools could AA leaders have used to increase their awareness of internal and external issues?...
What tools could AA leaders have used to increase their awareness of internal and external issues? ???ALASKA AIRLINES: NAVIGATING CHANGE In the autumn of 2007, Alaska Airlines executives adjourned at the end of a long and stressful day in the midst of a multi-day strategic planning session. Most headed outside to relax, unwind and enjoy a bonfire on the shore of Semiahmoo Spit, outside the meeting venue in Blaine, a seaport town in northwest Washington state. Meanwhile, several members of...
Delta airlines case study Global strategy. Describe the current global strategy and provide evidence about how...
Delta airlines case study Global strategy. Describe the current global strategy and provide evidence about how the firms resources incompetencies support the given pressures regarding costs and local responsiveness. Describe entry modes have they usually used, and whether they are appropriate for the given strategy. Any key issues in their global strategy? casestudy: Atlanta, June 17, 2014. Sea of Delta employees and their families swarmed between food trucks, amusement park booths, and entertainment venues that were scattered throughout what would...