Question

Match the chromatographic method to the protein characteristic it exploits/targets for purification. (5 pt) Chromatographic Method ____ Affinity ____ Cation Exchange ____ Hydrophobic Interaction ____ Anion Exchange ____ Size Exclusion Targeted Protein Characteristic A. Nonpolar protein regions B. Specific sequences, called tags C. Overall protein size D. Proteins with a net negative charge E. Proteins with a net positive charge

Answer 1

1.special sequence called tags -Affinity tags.these are used in purifying proteins from their source.

2.Cation exchange - protein with net positive charge.

3Hydrophobic interaction-involves purification of protein using Non Polar protein.

4.Anion exchange- protein with net negative charge.

5.size exclusion targeted protein characteristics-overall protein size. this method is also called gel filtration in which separation of molecules is done on the basis of size .