Volume of reactants should be kept constant to do a fair test and comparison of results.eg. If you are testing for the effect of increasing surface area, the volume of reactants should be kept constant.
Other reason is because reactions can be concentration
dependent. Side reactions producing by-products can be favored if a
reaction is performed either more dilute or more concentrated than
optimal. Yes, reaction volume does relate to concentration since
concentration is in g/mL.
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