Question

How would you prepare a 500mL of 100mM phosphate buffer at pH 7.2 if you have...

How would you prepare a 500mL of 100mM phosphate buffer at pH 7.2 if you have only solid Na3PO4 and 1M HCL and 1M NaOH (may not use all reagents). Find mass of Na2HPO4, Volume 1M HCL, and Volume 1M NaOH

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
How would you prepare 1 L of a 0.100 M phosphate buffer at pH 7.02 using...
How would you prepare 1 L of a 0.100 M phosphate buffer at pH 7.02 using crystalline Na2HPO4 and a solution of 1.0 M HCl?
You need to prepare a buffer for biochemistry lab. The required solution is 0.5Msodium phosphate, pH...
You need to prepare a buffer for biochemistry lab. The required solution is 0.5Msodium phosphate, pH 7.0. Use the Henderson-Hasselbalch equation to calculate the number of moles and grams of moobasic sodium phosphate ( NaH2PO4) and dibasic sodium phosphate ( Na2HPO4) necessary to make 1 liter of solution. The pKa for this buffer is 7.21
Would pls show all steps If 200 ml of 0.16 M phosphate buffer at pH 7.2...
Would pls show all steps If 200 ml of 0.16 M phosphate buffer at pH 7.2 has a new pH of 6.9 after some volume of 0.12 M hydrochloric was added. what volume of 0.12 M of hydrochloric acid was added.
A common biological buffer is a phosphate buffer which involves the equilibrium between H2PO4- and HPO4-2,...
A common biological buffer is a phosphate buffer which involves the equilibrium between H2PO4- and HPO4-2, with a pKa at 25 celcius of 6.86. Describe in words how you would prepare 500. mL of 0.250 M phosphate buffer at pH 7.2 given 500 mL each of 0.250 M NaH2PO4 and 0.250 M Na2HPO4 at 25 celcius.
You are trying to make 2 liters of 1 M phosphate buffer at pH 8. Calculate...
You are trying to make 2 liters of 1 M phosphate buffer at pH 8. Calculate how many grams of Na2HPO4 and NaH2PO4, respectively, are necessary. pKa = 7.2 MW Na2HPO4 = 141.96 g/mol MW NaH2PO4 = 119.98 g/mo
How would you prepare a .100 L of 50mM succinate buffer at pH 3.85 using solid...
How would you prepare a .100 L of 50mM succinate buffer at pH 3.85 using solid disodium suddinate, DI water and either 1.00 M NaOH or 1.00 M HCL. The formula weight of disodium succiante is 162 g/mol. pka1 = 4.21; pka2 = 5.64.
Describe in detail how you will prepare 500.0 mL of 0.040 M HEPES buffer at pH...
Describe in detail how you will prepare 500.0 mL of 0.040 M HEPES buffer at pH 7.45. In your answer, indicate how much(grams) of HEPES you will dissolve in what volume (mL), and what you will use to adjust the pH(NaOH or HCl).
How could you prepare a buffer of pH=4.50 from a 1M of NaOH and a 1M...
How could you prepare a buffer of pH=4.50 from a 1M of NaOH and a 1M of Acetic Acid (pka=4.63) describe how and do the calculations.
you need to prepare a buffer solution with a ph of 7.8. A lab mate tells...
you need to prepare a buffer solution with a ph of 7.8. A lab mate tells you to use N-2- hydroxyethylpiperazine-N'-2-ethanesulfonic acid(HEPES), a sulfonic acid with a pka of 7.5. since you know that the buffer pka should be close to the desired pH, this sounds good. You make the solution according to the following recipe, which is supposed to make 600 ml of a 0.5 M solution: dissolve 0.2 moles of HEPES in 400 ml of water. add 0.1...
Describe, stepwise, how you would prepare 0.5 L of a 0.15 M phosphate buffer, pH 12.5,...
Describe, stepwise, how you would prepare 0.5 L of a 0.15 M phosphate buffer, pH 12.5, using K3PO4·H2O (f.w. = 230.28) and K2HPO4 (f.w. = 174.18). Assume that after dissolving the salts your pH-meter reading is 11.9.
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT