A student measures the solvent height on a TLC plate as 11.5cm. Spot A was measured at a height of 4.7 cm. spot B at 2.3 cm, and spot C at 8.2. What are the Rf values for spots A, B,and C?
Retention factor (Rf) value for TLC is defined as,
Rf = Migration distance of spot of compound / Migration distance of solvent.
Migration distance of solvent = Solvent height = 11.5 cm
1) For compound A, migration distance = height of spot = 4.7 cm.
(Rf)A = 4.7 / 11.5
(Rf)A = 0.4087
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2) For compound B, migration distance = height of spot = 2.3 cm.
(Rf)B = 2.3 / 11.5
(Rf)B = 0.2
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3) For compound C, migration distance = height of spot = 8.2 cm.
(Rf)C = 8.2 / 11.5
(Rf)C = 0.7130
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