How would you prepare a .100 L of 50mM succinate buffer at pH 3.85 using solid disodium suddinate, DI water and either 1.00 M NaOH or 1.00 M HCL. The formula weight of disodium succiante is 162 g/mol. pka1 = 4.21; pka2 = 5.64.
Let ,
and
represents succinic acid, monosodium succinic acid and disodium
succinic acid respectively.
0.100 L of 50mM succinate buffer corresponds to
Let x moles of NaHA and 0.005-x moles of H2A are present.
Thus 0.00152 moles of NaHA and
moles of H2A are present.
0.00348 moles of H2A are obtained when 0.00348 moles of Na2A
react with
moles of HCl.
0.00152 moles of NaHA are obtained when 0.00152 moles of Na2A react with 0.00152 moles of HCl.
Total number of moles of Na2A required
The formula weight of disodium succiante is 162 g/mol.
Mass of disodium succinate
Total number of moles of HCl required
The volume of 1.00 M HCl required
The volume of 1.00 M HCl required
0.81 g of disodium succinate is dissolved in sufficient amount of water, 8.5 mL of 1.00 M HCl is added slowly and the mixture is shaken. Then it is diluted to mark in 100 ml standard volumetric flask.
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