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If you double the amount of enzyme present in your activity assay, should the activity double?...

If you double the amount of enzyme present in your activity assay, should the activity double? Why or why not?

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Answer #1

An enzyme (Catalase) is a biological catalyst. It can modify the rate of reaction without being changed itself. Every enzyme are capable of converting one kind of substrate molecule into one kind of product molecule, which forms the enzyme-substrate complex. If we increase the amount of enzyme present in our activity assay, it enhances the rate of reaction because there are a greater number of active sites accessible for the enzyme-substrate complex to be formed. If we double the amount of enzyme present in our activity assay, the activity should double.

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