Question

1. 210 nm, 220 nm and 254 nm are used together on the UV detector to detect species eluted out from the CE. Look up UV-Vis spectra of the three analytes and discuss which wavelength would be the most useful. Include the UV-Vis spectra of each chemical by drawing or printing out the spectra (include the reference). (No spectrum given.)

2. Describe the acidic and/or basic properties of the three analytes of aspirin, acetaminophen and caffeine (such as ionizable groups on each molecule and corresponding pKa/pKb). Can you predict the ionization state of each compound under the conditions of the experiment?

3. The method of standard addition is often used for samples with a complex matrix. The sample seems to have a complex matrix. Why is external calibration method instead of the method of standard addition, chosen for this experiment?

Answer 1