Question

Designer enzymes can be created

		using site-directed mutagenesis.
		by coupling natural evolutionary processes with rational design.
		using computer modeling to design a protein with the desired structure and activity.
		using any of the methods listed above.

Answer 1

answer : using computer modeling to design a protein with the desired structure and activity.

Recently, however, Stephen Mayo and Daniel Bolon of the California Institute of Technology in Pasadena unveiled a technique that allows them to bypass evolution and design an enzyme on a computer.As a starting point for the design, they picked a known protein backbone with no catalytic effect called thioredoxin. This was chosen because it is small (a chain of 108 amino acids) and stable. Then they used their program to modify thioredoxin's amino acid sequence until a new protein with enzymatic activity emerged.