Question

An enzyme-catalyzed reaction was carried out with the substrate concentration initially 3500 times greater than the Km for that substrate. After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 micro-moles. If, in a separate experiment, the same amount of substrate but six times as much enzyme had been combined in the same volume, how long would it take for the same amount (12 micro-moles) of product to be formed?

Answer 1

The enzyme converts 1% of substrate to product in 9 minutes

product concentration = 12 micromoles

the enzyme concentration is increased six times and substrate is also increased six times of initial amount.

The increase in concentration or amount of substrate will not affect the conversion rate. however the enzyme will do.

An enzyme can convert substrate to 12 micromoles in 9 minutes.

The same amount of product formed = 6 x time required initially

= 9 / 6 minutes

= 1.5 minutes