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Tosyl-L-phenylalanine chloromethyl ketone(TPCK) is an inhibitor of Chymotrypsin that binds in the active site and is...

Tosyl-L-phenylalanine chloromethyl ketone(TPCK) is an inhibitor of Chymotrypsin that binds in the active site and is covalently linked to His 57.

a) what type of enzymatic inhibitor is TPCK?

b) why is this inhibitor specific to chymotrypsin?

c)Briefly describe two aspects of the chymotrypsin mechanism that are affected by TPCK

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Answer #1

a) TPCK is a type of irreversible inhibitor. It will covalantly modify chymotrypsin and is hence irreversible.

b) The specifity is due to the presence of phenylalanine in TPCK. Chymotrypsin is specific to aromatic amino acid residues and due to the presence of the aromatic phenylalanine, TPCK is specific to chymotrypsin.

c) TPCK will react with His 57 residue of the enzyme. His 57 is a part of the catalytic triad and as a result this leads to the inactivation of the enzyme.

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