Question

In our column chromatography of spinach most students obtain three or four bands. However, a TLC...

In our column chromatography of spinach most students obtain three or four bands. However, a TLC of the same spinach extract can yield up to 7 spots on the TLC.

A. Explain why one can not isolate the same number of bands from the column as there are on the TLC?

B. How would you need to adjust the column chromatography experiment in order to isolate all the bands that are visible on the TLC?

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Answer #1

In Column we can separate tne members of a mixture, but it is neccesary to try different ratio of solvents, for example: Hexane:Acetate 90:10, 80:20, 70:30. In order to optimize separation. Another factor could be the size of column, it depends of mass of sample.

In TLC ratio of solvents is important too. And size is help full to separate more and more spots.

So if column have less bands you need to improve solvent ratio or change column size.

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