Question

a.You add 400 μL of ADH instead of 100 μL in ethanol and NAD+ mixture. The absorbance decreases; briefly explain this observation.

b. When you add 200 μL of NAD+ to the equilibrium mixture, you observe no change in the absorbance. Is this what you would expect? Give a reasonable explanation of the observation.

c.What do you expect to happen to the absorbance of the equilibrium mixture when you add a few crystals of sodium metabisulfite ? Explain.

Answer 1