Question

Two Fluorescent compounds A and B are present in a mixture. Using a spectrofluorimeter, how would...

Two Fluorescent compounds A and B are present in a mixture. Using a spectrofluorimeter, how would you determine the quantity of A in the Mixture if

- A absorbs radiation in a region where B does not?

- Both A and B absorb in the same region but A emits fluorescence at different wavelengths than B.

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Answer #1

- A absorbs radiation in a region where B does not?

- Both A and B absorb in the same region but A emits fluorescence at different wavelengths than B.

In both cases, we can measure the absorbance of A corresponding to for A.

We will prepare a series of standard solutions of known concentrations of A and measure absorbance of A corresponding to for A. Then we will prepare callibertion curve (absorbance vs concentration of standard solutions) for A. Then we measure he of A corresponding to for unknown solution of A. From this value and the caliberation curve we will determine the concentration of A in the unknown solution.

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