Assume you work as an engineer at a pharmaceutical
plant which produces therapeutic monoclonal antibodies (mAbs) from
Chinese Hamster Ovary (CHO) cells. You are tasked with inoculating
a 2,000 liter CHO cell reactor for mAb production. The final CHO
cell concentration must reach 10 g/L before harvesting and
downstream processing. Assume that the empirical chemical formula
of Chinese Hamster Ovary (CHO) cells is
CN0.25O0.51H2.02S0.007P0.016.
Calculate:
(a) the weight percent of phosphorus in CHO cells
(b) the amount of KH2PO4 you must add to the medium to achieve a
cell density of 10 g/L
a) we have empirical chemical formula
= CN0.25O0.51H2.02S0.007P0.016
calculate mass of empirical formula = 12 x 1 (C) + 14 x 0.25(N) + 16 x 0.5 (O) + 1 x 2.02(H) + 32 x 0.007(S) + 31 x 0.016(P) = 26.24g
mass of P in it = 31 x 0.016 = 0.496g
% = 0.496g/26.24g x 100 = 1.89%
b) we need total 2000L
and concentration = 10 g/L
total mass of CHO = 10 g/L x 2000 L = 20000g
1.89 % is P in it
= 20000g x 1.89/100 = 378.0 g
this P mass should come from KH2PO4
molar mass = 136.086 g/mol
31g in 136.086 g molecule
we need 378g P KH2PO4 = 136.086/31 x 378 = 1659.37g
Ans = 1659.37 g KH2PO4 needed
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